Forest Tree Improvement Research

1. Pollen and Pollination of Azadirachta excelsa (Jack) Jacobs.
2. Pollen of Teak (Tectona grandis L.f.): Morphology and Viability of Pollen Grain
3. Preliminary Studies on Insect Visitors to Azadirachta excelsa (Jack) Jacob Inflorescence
4. Results of Acacia crassicarpa Provenance Trial at Kanchanaburi Province
5. Evaluation on Growth of Teak International Provenance Trial at Dong–Lann Teak Seed Orchard, Khon Kaen Province
6. Flower and Pollen Morphology of Dipterocarpus alatus Roxb.
7. Germination of Teak (Tectona grandis L.f.) Pollen from Different Crown Directions

By… Amnuayporn Choldumrongkul, Sudarath Visutithepkul and Pongtorn Bannasopits

ABSTRACT

Pollen and pollination of Azadirachta excelsa (Jack) Jacobs. were carried out during January to June 1995. Four trees of A. excelsa in Nah Rai village, Thumbon Mamuang Song Thon, Muang District, Nakorn Sri Thammarat Province were used for this experiment.

By using a light microscope and a scanning electron microscope, it was found that pollens are single grain with 4-colporate ora lalongate, prolate-spheroidal shape (polar axis 31-40 micron), amb circular and perforate exine. Result from pollen test in 10% sucrose showed that the pollens attached to the style could germinate better than those from the dehisced anther. By staining with decolorize aniline blue and viewing with a fluorescence microscope, it was clear that only pollen tubes originated from the pollens attached to the style were only observed, but not from the pollens on the stigma. According to tetrazolium test, the pollens detected from the indehisced anther showed better staining effect in 1% than in 0.5% tetrazolium, while the pollens from the dehisced anther did not react with the chemical. A. excelsa was found to be a cross-pollinated species because seeds were observed only on the uncovered inflorescence while there was no seed on the covered inflorescence with a cotton bag over a wire frame.

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เอกสารสมทบการประชุมการป่าไม้แห่งชาติ ประจำปี 2538 ระหว่างวันที่ 20-24 พฤศจิกายน 2539,
มหาวิทยาลัยเกษตรศาสตร์ กรุงเทพฯ. หน้า 11-25.

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By… Amnuayporn Choldumrongkul, Pisal Wasuwanich and Wiroj Ratanaporncharoen

ABSTRACT

Morphology and viability of the pollen grains of Teak (tectona grandis L.f. ) were carried out in Microtechnique Laboratory, Silviculture Research Division, Royal Forest Department, Bangkok during May to October 1995. Three T. grandis trees were selected and their inflorescences with pollens were collected during 08:00–11:00 a.m. of the day.

By using a light microscope and a scanning electron microscope, it was found that the pollen is single grain, with 3-colporate, amb circular and reticulate exine. The pollen grain of teak has two has two shapes. One is prolate-spheroidal with polar axis of 30.53 m (22.50 to 37.50 m ) and equatorial axis of 28.79 m (20 to 35 m ). Another is perprolate with polar axis of 25.41 m (21.25 to 29.75 m ) and equatorial axis of 16.30 m (10 to 26.25 m ). The ratio between the number of the prolate-spheroidal and the perprolate forms are 3:2. Brewbaker’s pollens germination medium with 3% sucrose is the best media for the pollen germination test. However, only some of the prolate-spheroidal grains could germinate and was considered, as fertile pollens while the perprolate grain did not germinate.

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รายงานการสัมมนาวนวัฒนวิทยา ครั้งที่ 6 ระหว่างวันที่ 21-24 พฤษภาคม 2539 อุดรธานี, หน้า 33-45.

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By… Amnuayporn Choldumrongkul and Petcharee Seng-sim

ABSTRACT

The study on insect visitors to Azadirachta excelsa (jack) Jacob is a priliminary study to determine insect species and quantity of each species that visit inflorescence of A. excelsa so as to provide guidelines and basic data for further study on insect pollinators of A. excelsa. This study was carried out in Amphur Muang, Nakhon Si Thammarat Province during10 February to 11 March 1993. Three of A. excelsa trees were randomly selected and the investigated inflorescences of each tree were about 25–30 m from the ground. Insect capture was done with a sweep net at the radius of 50 cm from the inflorescences during 08.00–10.00 hrs and 14.00–16.00 hrs. The result of this study indicate that insect visiting the inflorescences of A. excelsa belong to three Orders Hymenoptera, Coleoptera and Diptera. The Hymenoptera was mostly found and the stringless bee (Apis indica F. and Apis sp.). Ronalia sp., Vespa sp., Sceliphron sp. ants and some unidentified of different Orders were also observed irregularly and in low number.

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รายงานการประชุมวิชาการป่าไม้ ประจำปี 2536 ระหว่างวันที่ 20-24 ธันวาคม 2536 กรุงเทพฯ, หน้า 97-104.

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By… Panida Roongrattanakul, Vitoon Luangviriyasaeng and Kiatkong Pitpreecha

ABSTRACT

Provenance trial of Acacia crassicarpa at Lumpao-LumSai experiment station, Kanchanaburi province consisted of 9 provenances from 2 regions as Papua New Guinea region (PNG) and Queensland region (QLD).

Results of height growth at 5 years of age showed significant difference at P>0.05 between two regions but it did not significant between provenance within region. The mean height of PNG was higher than QLD which Bimadebun village provenance (CSIRO seedlot 16598 PNG) is the highest height growth.

Diameter at breast height (DBH) was not significant difference (P>0.05) between two regions and between provenances within region. The Claudie river provenance (CSIRO seedlot 17944 QLD) was the poorest of height and DBH.

Three phenology characteristics (stem form, branching habits and fruiting and health) was assessed by scoring system which were not significant difference (P>0.05) between regions for most characteristic except stem form, stem straightness (highly significant at P<0.01). It was not significant difference between provenance within region. The average phenology score of QLD was higher than PNG which showed that stem straightness of QLD was better than PNG. Less multiple stems were founded in this trial (4.5%) and branching habits mean score were higher than grand mean. The phenology of fruiting and health showed that most trees in the trial had not fruited yet and had free from insects and diseases.

Keywords: Acacia crassicarpa, Provenance trial, Stem from, Branching habits

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รายงานวนวัฒนวิจัย ประจำปี 2542 ส่วนวนวัฒนวิจัย สำนักวิชาการป่าไม้ หน้า 148-161

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By... Thavee Chairuangsirikul

ABSTRACT

Twenty–five years old of Teak International Provenance Trial (IP 038) established at Dong–Lann Teak Seed Orchard, Chum Phae district, Khon Kaen province (outside the teak region) was assessed and evaluated for their growth in 1998. The trial consisting of 25 provenances, i.e. 5 provenances from Indonesia, 4 provenances from Laos, 9 provenances from India, 1 provenance from Africa (land race) and 6 provenances from Thailand, It was established in 1973 with 4 block, 7x7 trees/plot and 4x4 meters spacing. The results were shown as below.

The average value of dbh, SD., and heritability (h²) of 25 years old trees in Teak International Provenance Trial were 28.08 cm., 2.26, 0.35 respectively. The trees from provenance 3048 (Indonesia) had highest genetic value (G) (6.29%) and provenance 3034 (India) (4.81%), 3049 (Indonesia) (4.44%) and 3040 (Thailand) (3.92%) were second, third and fourth respectively.

The average value of Total height, SD., and heritability (h²) were 19.38 m., 1.01 m and 0.33 m respectively. The trees from provenance 3040 (Thailand) had highest genetic value (G) (3.65%) and provenance 3047 (Indonesia) (2.29%) and 3016 (India) (2.08%) were second and third respectively.

The growth index (H) (by economic weight of dbh: total height = 0.67: 0.33) of teak from provenance 3048 (Indonesia) was highest (4.41%) and provenance 3040 (Thailand) (3.83%) and 3049 (Indonesia) (3.25%) were second and third respectively.

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รายงานวนวัฒนวิจัย ประจำปี 2542 ส่วนวนวัฒนวิจัย สำนักวิชาการป่าไม้ หน้า 137-147

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By… Amnuayporn Choldumrongkul

ABSTRACT

Study on flower and pollen morphology of Dipterocarpus alatus Roxb. was carried out in March 1997. Three trees of C. alatus in Pak Tong Chai District, Nakhon Ratchasima Province were used for this study. By using a light microscope and a scanning electron microscope, it was found out that flower is perfect, comprising of calyx, corolla, androecium and gynoecium. Pollen is single grain, 3–colpate, prolate shape with polar axis at 67.50 m m (60–75 m m) and equatorial axis at 42.50 m m (35–50 m m) and rugulate exine.

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รายงานการสัมมนาทางวิชาการเรื่อง “ไม้ยางนาและไม้ในวงศ์ไม้ยาง” ระหว่างวันที่ 17-18 พฤศจิกายน 2542
ณ มหาวิทยาลัยเกษตรศาสตร์ กรุงเทพฯ. หน้า 199-203.

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By… Amnuayporn Choldumrongkul, Silapachai Pipitvitaya and Wiroj Ratanaporncharern

ABSTRACT

Germination of Teak (Tectona grandis L.f.) pollens collected from different crown directions (north, south, east, west and in the central part of the crown) were investigated. The pollens were collected from 23 years old teak trees grown in Mae Tha Seed Orchard, Lampang province, in July, August and September 1997. The test was done in Brewbaker’s pollen germination media within 24 hours after collection. The result revealed that differences in germination percentages of pollens from the directions were statistically significant in July and highly statistically significant in August and September. Germination percentage of pollen collected from southern direction was lowest. However, no significant difference of germination percentage of pollen collected from all directions in different months was observed.

Keywords : pollen germination, crown direction, Teak (Tectona grandis L.f)

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วิทยาสารเกษตรศาสตร์ สาขาวิทยาศาสตร์ ปีที่ 33 ฉบับที่ 3 หน้า 330-334

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